Alignment
The color of the Sequence Alignment bar is determined by the Alignment Identity %:
- 100%: Dark Red
- 99 - 95%: Orange
- 94 - 90%: Yellow
- 89 - 80%: Green
- 79 - 60%: Teal
- Below 60%: Blue
Sequence mismatches between the query and the subject are highlighted in blue, and partial sequence mismatches (i.e., mismatches within the same amino acid family) between the query and subject are highlighted in yellow and designated by + signs.
Subject
The Subject tab displays sequence-level detail for the subject (hit) sequence to which the query aligned:
- Sequence Length
- CAS Registry Number®: Get registry numbers (RNs) for a particular sequence result in CAS Registry:
- Click the Get CAS Registry Number button to get the single RN.
- If multiple RNs, click the Get CAS Registry Numbers button to get all RNs (up to 5000).
- GenBank Accession No: Get accession numbers for a particular sequence result:
- Click the Get GenBank Accession Number button to get the single GenBank accession number.
- If multiple accession numbers, click the Get GenBank Accession Nos. button to get all numbers (up to 5000).
- Organism: The NCBI Organism name associated with the sequence.
- Sequence: To download the sequence in either .txt or .FASTA format, click the Download Sequence button, and then select the appropriate file type.
Note: You have the option to save either action as a script.