Search Sequences - BLAST

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  1. Click My Files, and then select CAS Sequences.

    _STNext-MyFilesMenu-Biosequences.png

  2. On the CAS Sequences page, click the New Search button.

    _STNext-MyFiles-BiosequenceSearch-NewSearchButton.png

  3. Click the BLAST tab.

    _STNext-MyFiles-BiosequenceSearch-BLAST-BLAST_Tab.png

  4. Import (.txt files only) or enter the query sequence.

    _STNext-MyFiles-BiosequenceSearch-BLAST-ImportOrEnterSequence.png

    Note: If importing a sequence, the .txt file's name replaces the auto-generated text in the Name field.

    _STNext-MyFiles-BiosequenceSearch-ImportBiosequenceFilePopup.png

    _STNext-MyFiles-BiosequenceSearch-ImportedFileName.png

  5. Select the desired search options:
    • Sequence type
    • Search within

      _STNext-MyFiles-BiosequenceSearch-BLAST-SearchOptions.png

      Advanced Sequence Search can be used to increase the speed of the search, adjust for the length of the query sequence, or tune the sensitivity of the search for finding sequences that are more distantly related to the query or finding nearly exact sequences.

      _STNext-MyFiles-BiosequenceSearch-BLAST-Advanced.png

  6. Click the Run Search button. Note: If the imported or entered sequence is in an invalid format or contains invalid characters, an error message displays.

    _STNext-MyFiles-BiosequenceSearch-BLAST-RunSearchButton.png

You will then see your search is in progress; when complete, click the View Results button.

_STNext-MyFiles-BiosequenceSearch-SearchInProgress.png

_STNext-MyFiles-BiosequenceSearch-BLAST-ViewResultsButton.png